Experiment 3: Purification of a His-Tagged Fatty Acid Bind Protein (FABP5)

On a seperate doc
– eRRORS within the experimnet 
– Within the context of the purification that you performed, discuss 3 variables that one couldmanipulate to improve the purity and 3 variables that one could manipulate to improve the yieldof the his-FABP5 protein. Consulting the literature, describe an additional experimental step(s)that one could employ to improve the purification procedure. Outline a purification procedure(from the literature) that was employed for any of the other fatty acid binding proteins such asFABP3 or FABP7 (just a few sentences listing the steps).